Development of Trypan Blue Polymer Film Based Electrochemical Sensor for the Determination of Dopamine and its Simultaneous Detection in Presence of Ascorbic Acid and Uric acid: A Voltammetric Method

This paper deals with the development of an electrochemical sensor base on Trypan Blue (TB) towards the determination of Dopamine (DA). An electrochemical sensor was fabricated by the electropolymerization of TB onto the Carbon paste electrode (CPE) using cyclic voltammetry (CV) technique. The polymerized TB modified CPE (Poly(TB)MCPE) was characterized using Electrochemical impedance spectroscopy (EIS) and CV techniques. The sensor Poly(TB)MCPE showed good sensitivity and catalytic activity in comparison with bare CPE. Electrochemical behaviour of Dopamine (DA) was investigated in 0.1 M PBS in alkaline medium (pH 8.0) using Poly(TB)MCPE. Moreover, simultaneous determination of DA in presence of Ascorbic acid (AA) and Uric acid (UA) was examined using Differential pulse voltammetry (DPV) technique. Several kinetic parameters such as diffusion coefficient (D0), area of the electrode (A), surface concentration of modifier on Poly(TB)MCPE (Γ), limit of detection (LOD) and limit of quantification (LOQ) were calculated. KeywordsDopamine, Trypan blue, Cyclic voltammetry, Differential pulse voltammetry Electrochemcal impedance spectroscopy, Electrochemical sensor Analytical & Bioanalytical Electrochemistry © 2014 by CEE www.abechem.com Anal. Bioanal. Electrochem., Vol. 6, No. 4, 2014, 485-500 486